Neuromodulation of Ion Channels and Calcium Signaling in T Lymphocytes
Annual rept. 1 Feb 1988-31 Jan 1989
CALIFORNIA UNIV IRVINE DEPT OF PHYSIOLOGY AND BIOPHYSICS
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Ion channels play a crucial role during early periods of lymphocyte activation by mitogens. This project seeks to define the molecular substrates of neural modulation of immunity, using a combination of patch clamp methods to examine ion channels in lymphocytes, and fluorescence imaging to monitor changes in intracellular Ca2. Our hypothesis is that neurotransmitters and peptides may influence functional responses of the immune system by modulating properties of ion channels, which in turn regulate several aspects of lymphocyte activation and effector function. Over the past year we have constructed two experimental setups to monitor Ca2 inside cells using fura-2, a dye which changes its fluorescence excitation properties when CA2 binds. We have also further characterized the diversity of channels which occurs in T lymphocytes and demonstrated that the distribution of K channels is subset-specific. These experiments have expanded the list of potential substrates for neural modulation of immunity, and have provided the technical framework for studying ion channels responsible for Ca2 signalling, a cellular response to mitogens crucial for activation. Keywords Calcium, Potassium, Ion channels.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research