Accession Number:

ADA202780

Title:

Effects of Simultaneous Radiofrequency Radiation and Chemical Exposure of Mammalian Cells. Volume 2

Descriptive Note:

Final rept. 2 Jan 1984-31 Dec 1986

Corporate Author:

TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO DEPT OF RADIOLOGY

Report Date:

1988-07-01

Pagination or Media Count:

84.0

Abstract:

A circulating water-bath exposure system has been designed for in vitro radiofrequency radiation RFR exposure studies in the 915 to 2450 MHz range. A continuously rotating Styrofoam float, holding ten T-25 tissue culture flasks, averages out field heterogeneity and allows mixing in the medium in the flasks. The presence of cells in the exposure flask as attached monolayer or cell suspension did not result in an SAR different from that measured in the medium without cells present. We have posed two hypotheses relative to the mutagenic activity of RFR a that RFR by itself is genotoxic to mammalian cells in vitro and b that a simultaneous exposure of mammalian cells to RFR during treatment with a genotoxic chemical will result in an alteration of the genotoxic activity of the chemical alone. RFR exposure alone at moderate power levels which resulted in a temperature increase in the cell culture medium of no more than 3 C is not mutagenic. during simultaneous treatment, the RFR does not affect either the inhibition of cell growth or the extent of mutagenesis resulting from the chemical treatment alone. The same two hypotheses were explored for induction of sister chromatid exchanges SCEs and chromosome aberrations in Chinese hamster ovary cells which were similarly exposed to RFR and chemicals. The chemicals studied for SCE induction were mitomycin C MMC and Adriamycin. Keywords Microwaves.

Subject Categories:

  • Radiobiology
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE