Accession Number:

ADA202381

Title:

Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-aged and Aged Organophosphoryl Conjugates of AChE

Descriptive Note:

Annual summary rept.

Corporate Author:

ISRAEL INST FOR BIOLOGICAL RESEARCH NESS ZIONA

Personal Author(s):

Report Date:

1985-09-01

Pagination or Media Count:

85.0

Abstract:

The objective of this project is to detect and characterize, by nmr and fluorescence spectroscopy, differences in the conformation of non-aged and aged organophosphoryl OP conjugates of acetylcholinesterase AChE and alpha- chymotrypsin a-Cht. Two series of novel fluorescent OP inhibitors were prepared, containing either pyrenebutyl or dansylaminoethyl moieties these inhibitors were used to obtain homologous pairs of non-aged phosphorylated conjugates of alpha-Cht. The techniques employed to characterize these conjugates, and to detect differences between homologous aged and non-aged conjugates, included kinetic measurements of inhibition, reactivation, and aging, 31P-nmr spectroscopy, and circu-larly polarized luminnescence CPL. The main findings and principal conclusions are summarized.

Subject Categories:

  • Organic Chemistry
  • Physical Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE