Accession Number:

ADA202171

Title:

Density Gradients for Isolation of Mononuclear Blood Cells for Magnesium Analysis by Electron Probe X-Ray Microanalysis

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1988-01-01

Pagination or Media Count:

6.0

Abstract:

Magnesium in peripheral mononuclear blood cells MBC may be a useful indicator of intracellular Mg status. We present a mononuclear blood cell MBC isolation method for magnesium analysis by electron probe X-ray microanalysis. We compare the inorganic elemental composition of individual MBC isolated by either arabinogalactan Stractan or conventional Ficoll-Hypaque density gradients. We find that the MBC isolated with Stractan have the expected cellular element composition, but MBC isolated Ficoll-Hypaque are contaminated with iodine and sodium. We discuss the source and significance of iodine and sodium contamination of Ficoll-Hypaque isolated MBC and recommend the Stractan separation method for electron probe X-ray microanalysis. Key Words Mononuclear blood cells Density gradient separation Electron probe Arabinogalactan Reprints.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE