Accession Number:

ADA200488

Title:

Comparative Testing of Monoclonal Antibodies against Plasmodium falciparum Sporozoites for ELISA (Enzyme-Linked Immunosorbent Assay) Development

Descriptive Note:

Journal article

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Report Date:

1987-01-01

Pagination or Media Count:

8.0

Abstract:

Ten monoclonal antibodies developed against Plasmodium falciparum sporozoites at four institutions were evaluated for use in an enzyme-linked immunosorbent assay ELISA. Four of the antibodies were eliminated because of their low sensitivity or requirement for high concentrations of capture antibody, with an additional four were rejected because they exhibited cross- reactivity with P. berghei sporozoites. Of the two remaining monoclonal antibodies, that designed 2A10 had the highest sensitivity, a requirement for lower concentrations of capture antibody, and had been tested successfully against sporozoites from a wider range of geographical areas than the others. Use of this monoclonal antibody in a standardized ELISA method gave a test ten times more sensitive than previously reported for P. falciparum sporozoites an its detection limit was less than 100 sporozoites per mosquito. Reprints.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE