Developmental Toxicity Potential of Nitroguanidine in Rabbits
Developmental toxicity study
LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
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The potential of nitroguanidine to produce developmental toxicity was evaluated in pregnant New Zealand White rabbits. Nitroguanidine, suspended in 1 carboxymethylcellulose, was administered at doses of 0, 100, 316, and 1000 mg kgday by oral gavage on Days 6 through 18 of gestation. Fetuses were delivered by cesarean section on Day 29, weighed and examined externally. The soft tissues were examined while the body was being eviscerated for subsequent processing in alizarin red stain for skeletal examination. Ten dams in the 1000-mgkgday group died or were terminated in a moribund condition following a generalized failure to thrive. The dams administered 1000 mgkgday nitroguanidine exhibited weight loss and decreased food consumption. Signs of developmental toxicity associated with nitroguanidine administration were an increased incidence of resorptions in all dose groups. Fetuses in the 1000-mgkgday group were lighter in weight and had an increased incidence of retarded ossification of the sternebrae, olecranon, patellae, and phalanges. There were no dose-related malformations. On the basis of these findings, we concluded that nitroguanidine had no teratogenic potential but does have the potential to cause developmental toxicity. Keywords Teratology, Triple base propellants, Teratogenic compounds.
- Animal Husbandry and Veterinary Medicine