Accession Number:

ADA200140

Title:

Determination of Dihydroqinghaosu in Blood by Pyrolysis Gas Chromatography/Mass Spectrometry

Descriptive Note:

Manuscript

Corporate Author:

WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF EXPERIMENTAL THERAPEUTICS

Report Date:

1988-01-15

Pagination or Media Count:

7.0

Abstract:

A sensitive and specific method using packed or capillary column gas chromatographymass spectrometry GCMS has been developed for the quantitation of the major active metabolite dihydroqinghaosu DQHS of the antimalarial drug artesunic acid in blood. Quantification is achieved with an internal standard method, petroleum ether or n-butyl chloride extraction of blood and gas chromatographymass spectrometry analysis of the extract with selected ion monitoring. Since DQHS is thermally unstable, the method is based on the observations that at high injector temperature and specific GC conditions, DQHS is pyrolyzed to 2S, 3R, 6R-2-3-oxobutyl-3-methyl-6-R-2- propanalcyclohexanone. Quantitation of this cyclohexanone pyrolysis product by GCMS with selected ion monitoring precisely and accurately reflects DQHS blood levels and calibration curves are linear in the range of 10-1000 ngmL. The method is currently being used for pharmacokinetic and metabolism studies in animals. Reprints.

Subject Categories:

  • Biochemistry
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE