Detection of Heat-Stable Enterotoxigenic Escherichia coli by Hybridization with an RNA Transcript Probe
ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES APO SAN FRANCISCO 96346
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Enterotoxigenic Escherichia coli ETEC is usually identified by testing isolates for enterotoxin production in bioassays or serological tests. Alternatively, ETEC can be identified by detecting the genes encoding these enterotoxins by DNA hybridization, a method which has been particularly useful in detecting ETEC in large numbers of specimens. Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. Radiolabeled enterotoxin gene RNA transcripts are easier to prepare and avoid the preparation of cloned DNA probes that can be nonspecific if they contain cloning vector DNA. Keywords Reprints.
- Genetic Engineering and Molecular Biology