Accession Number:

ADA200068

Title:

HeLa Cell Adherence and Cytotoxin Production by Enteropathogenic Escherichia coli Isolated from Infants with Diarrhea in Thailand

Descriptive Note:

Journal article

Corporate Author:

ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES APO SAN FRANCISCO 96346

Report Date:

1987-08-01

Pagination or Media Count:

6.0

Abstract:

Enteropathogenic Escherichia coli EPEC strains from hospitalized infants with diarrhea in Thailand were examined for HeLa cell adherence and cytotoxin production. Of 101 strains examined, 56 adhered to HeLa cells in a localized pattern LA, 27 adhered in a diffuse pattern DA, and 18 did not adhere. All 56 LA EPEC strains were OK serotype 0119K69. A total of 20 83 of 24 EPEC 086K61 strains and 7 38 of 19 EPEC strains belonging to six other OK serotypes exhibited DA. All LA EPEC strains hybridized with a DNA probe for genes encoding EPEC adherence factor, whereas none of the 27 DA or 18 nonadherent EPEC strains hybridized with EPEC adherence factor probe. Sonic extracts of 57 58 of 98 EPEC strains tested at a dilution of 1100 caused greater than 25 mortality of HeLa cell monolayers. A total of 50 88 of 57 cytotoxic sonic extracts were inhibited to various degrees by a 1500 dilution of polyclonal rabbit antisera to purified Shiga toxin. The mean percent inhibition of cytotoxic sonic extracts by anti-Shiga toxin was 67 range, 29 to 89. Fifty percent 38 to 56 of LA EPEC strains, fifty-two percent 14 of 27 of DA EPEC strains, and fifty-three percent 8 to 15 of nonadherent EPEC strains produced Shiga-like toxins. Both adherence and low levels of cell- associated cytotoxins were identified in EPEC strains from Thailand, but there did not appear to be an association between these two factors. Reprints.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE