Microcomputer Software and Interface for Control of a Microscope Scanning Stage
Rept. no. 8
NAVAL MEDICAL RESEARCH INST BETHESDA MD
Pagination or Media Count:
Motorized scanning stages are valuable in microscopy systems that employ digital image analysis and for the development of semiautomatic computer- assisted microscope systems the development of standard software tools to control such stages will facilitate their integration into a variety of computer-based systems. A set of Microsoft BASIC and Turbo PASCAL programs that interfaces a microprocessor-controlled stepper motor microscope stage MDACE 1000 to an IBM PC or PC-AT or compatible microcomputer via a serial interface RS-232 is described. These programs can be integrated into other software written in either BASIC or PASCAL, or used via a menu program that directs the routines to control scanning patterns and to locate the microscope stage to a selected area of the slide. Coordinates of significant events on a slide can be stored on a disk file to allow future examination. The software and interface also provide control of a filter wheel in the microscope for use in multicolor fluorescence assays. Keywords Reprints, Image processing, Man computer interface.
- Computer Programming and Software