Field Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) for Plasmodium falciparum Sporozoite Detection in Anopheline Mosquitoes from Kenya
ARMY MEDICAL RESEARCH UNIT-KENYA APO NEW YORK 09675
Pagination or Media Count:
An enzyme-linked immunosorbent assayELISA using a monoclonal anti- body that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88 of 44 sporozoite-positive gland dissections were P. falciparum. ELISA tests on body portions showed that 29.5 of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections.This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions. Keywords Reprints Test method.
- Test Facilities, Equipment and Methods