A Procedure For the Purification of Rabbit Sperm Cells and the Separation of Sperm Heads and Tails
Technical rept. Jan 1985-Aug 1986
CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
Pagination or Media Count:
Sperm cells collected from mammals by electroejaculation or with an artificial vagina are contaminated by secretions and fluids from the accessory glands, cell debris, and particulate matter such as prostatic vesicular bodies. Seminal plasma is commonly removed by several washes with buffer or medium and centrifugation. Sperm cells washed by the procedure are unsuitable for many biochemical studies because traces of seminal plasma remain, and particulate matter and cell debris are still present. Accessory gland secretions and particulate matter can be effectively removed by centrifugation of sperm cells through a discontinuous sucrose density gradient. Sperm cells washed in the manner can be used for many biochemical studies. The study of neurotransmitter receptors in rabbit sperm cells required large quantities of sperm cells and separated sperm heads and tails. A procedure, based on discontinuous sucrose density centrifugation, was developed for the large scale preparation of uncontaminated rabbit sperm cells and sperm heads and tails.