Accession Number:

ADA199681

Title:

Investigating the Structural Bases of Voltage-Gating Model Channels by Using Perfectly Aligned Multilayer Samples

Descriptive Note:

Annual rept. Oct 1987-Sep 1988

Corporate Author:

RICE UNIV HOUSTON TX DEPT OF PHYSICS

Personal Author(s):

Report Date:

1988-09-20

Pagination or Media Count:

8.0

Abstract:

One dimensional quasi crystals of perfect multilayers, in which ion channels are uniformly oriented within parallel membranes, can be used to study the structural base of channel conductivities. We have developed 1 the techniques for preparing such multilayer samples and 2 the spectroscopic methods circular dichroism and x-ray diffraction for extracting structural information from these samples. The sample variables include electric field, water content, ion concentrations, etc. We have observed conformation changes of alamethicin with water content, a result in favor of the barrel model rather than the flip-flop model for the channel. Our goal is to probe the conformation changes of the channels as we vary the sample variables, in order to elucidate the molecular mechanisms of voltage gating. Keywords Alamethicin Melittin Gramicidin Orientation Dependent Circular Dichroism X-ray Diffraction Neutron Scattering Perfectly Aligned Multilayers.

Subject Categories:

  • Electrooptical and Optoelectronic Devices
  • Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE