Accession Number:

ADA199650

Title:

Use of Monoclonal Antibodies to Study the Structural Basis of the Function of Nicotinic Acetylcholine Receptors on Electric Organ and Muscle, and to Determine the Structure of Nicotinic Acetylcholine Receptors on Neurons

Descriptive Note:

Annual rept. 15 Feb 86-14 Feb 87

Corporate Author:

SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA RECEPTOR BIOLOGY LAB

Personal Author(s):

Report Date:

1987-03-16

Pagination or Media Count:

61.0

Abstract:

The structure of muscle-type nicotinic acetylcholine receptors from Torpedo electric organ was studied. These are composed of four kinds of subunits termed alpha, beta, gamma and delta in order of increasing molecular weight. The alpha subunits form the acetylcholine binding sites. The acetylcholine binding site was identified. The main immunogenic region was shown identified. Epitopes for several monoclonal antibodies to alpha subunits were mapped, and the transmembrane orientation of these sequences was determined. Keywords Molecular structure. KT

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE