Accession Number:

ADA199452

Title:

Effects of Immunomodulatory Drugs on T Lymphocyte Activation and Function

Descriptive Note:

Annual rept. 15 May 1987-14 May 1988

Corporate Author:

SCRIPPS CLINIC AND RESEARCH FOUNDATION LA JOLLA CA DEPT OF BASIC AND CLINCALRESEARCH

Personal Author(s):

Report Date:

1988-06-15

Pagination or Media Count:

20.0

Abstract:

During this second reporting year the following drugs were tested for their immunomodulatory actions CL246, FK565, OK432, AVS-1300, AVS-2149, and AVS-1761. The drugs were tested for their effects on the proliferative activity of human peripheral blood lymphocytes induced by the mitogenic lectin Concanavalin A Con A. All of the drugs, except FK565, inhibited lymphocyte proliferation in a dose dependent manner. The potency of inhibition varied among the drugs. We tested whether the inhibition of proliferation was secondary to interfering with the production of the soluble mediators IL1 and IL2 by the drugs. An explanation of the effects of these drugs on lymphocyte proliferation could had been interference with the expression of critical cell surface receptors. To this end, lymphocytes were stimulated with Con A in the presence of drug and the expression of specific receptors was assessed by specific monoclonal antibodies and indirect immunofluorescence. These studies indicate that the above drugs have immunoregulatory effects on human lymphocyte proliferation which can be explained due to secondary effects on the production of IL1 and IL2, as well as, the expression of important cell surface receptors. Finally, of all the drugs, the most remarkable inhibitory effects were displayed by CL246 while the most remarkable potentiating effects were displayed by FK565. Keywords Immunochemistry, Immunomodulators, Receptor sites, Active sites, Cytotoxic cells.

Subject Categories:

  • Biochemistry
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE