Accession Number:

ADA199421

Title:

Cloning Sequencing and Structural Manipulation of the Enterotoxin D and E Genes from Staphylococcus aureus

Descriptive Note:

Annual summary rept. 1 Nov 1986-30 Oct 1987

Corporate Author:

KANSAS STATE UNIV MANHATTAN DIV OF BIOLOGY

Personal Author(s):

Report Date:

1988-06-01

Pagination or Media Count:

25.0

Abstract:

We cloned the gene for exfoliative toxin A from the chromosome of S. aureus expression vector gamma gtll. The DNA fragment expressing exfoliative toxin A ETA was subcloned in E. coli and unlike the etb gene was fully expressed. Both eta and etb were completely sequenced and the coding regions for both toxins were compared. Significant stretches of both DNA and protein sequence similarities were found. We also report the sequencing of the enterotoxin D gene entD of S.aureus 485. The protein sequence of SED is highly similar to the other enterotoxins especially SEA and streptococcal pyrogenic exotoxin A. The phage attachment sites of the lipase inactivating bacteriophage L54a have been identified, cloned and sequenced. A core sequence of homology within the lipase gene and the phase genome serve as sites for reciprocal recombination. The resultant integrated phage is flanked by this core sequence. Lastly, we were also able to map the bacteriophage functions necessary and sufficient for integration and excision of the phage.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE