Accession Number:

ADA199364

Title:

Mechanism of Cytotoxicity of the Aids Virus, HTLV-III/LAV

Descriptive Note:

Annual rept. 16 Mar 1987-15 Mar 1988

Corporate Author:

WASHINGTON UNIV ST LOUIS MO

Personal Author(s):

Report Date:

1988-04-19

Pagination or Media Count:

12.0

Abstract:

The study was undertaken in order to devise a quantitative assay of human immunodeficiency virus type 1 HIV-1 load in patients tissues. No methods are currently available which are both sensitive and specific for this purpose. Our method utilizes blood mononuclear cell or tissue DNA. HIV-1 sequences are amplified by the primer chain amplification reaction PCR technique. The reaction products are detected and quantitated by polyacrylamide gel electrophoresis, ethidium bromide stain, and Southern blot hybridization. This method has proven successful using a single set of primers in detection of HIV-1 DNA sequences from 10 of 12 HIV-1 infected individuals, and 0 of 10 uninfected individuals. Methods of quantitation of using standard curves with defined amounts of HIV-1 DNA sequences and internal controls are under investigation. In addition, we have developed a sensitive and specific assay from HTLV-1 and HTLV-II DNA sequences using the same methodology and we have successfully applied it to the evaluation of blood samples from asymptomatic HTVL-1 infected individuals and individuals with adult T cell leukemia-lymphoma. Keywords Immunosuppression Diagnosis medicine.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE