Production of Spores of PA3679 of High Heat Resistance and High Yield in a Biphasic Beef Heart Infusion Medium
Final progress rept. Oct 1986-Feb 1988
ARMY NATICK RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA
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Spores of Clostridium sporogenes strain PA3679, are among the most resistant of food spoilage organisms, and are used as nontoxic surrogate test organisms in lieu of Clostridium botulinum, to evaluate thermal processing in low-acid canned foods. It is often necessary to challenge a particular product with an inoculated pack study in order to confirm preliminary results obtained with resistance studies conducted in buffer or in food materials with subsequent recovery in optimal growth medium, or on a small scale using small quantities of food materials. The capability of producing clean spores of PA3679 having the required resistance in order to conduct an inoculated pack study is therefore of great importance. A method is described for the production of spores of Clostridium sporogenes strain PA3679 using a biphasic beef heart medium. These spores were produced in high yield and exhibited a higher heat resistance than any reported to date in the literature. Keywords Anaerobic bacteria Food processing.
- Food, Food Service and Nutrition