Structure and Functional Studies on Dengue-2 Virus Genome
Annual rept. 1 Sep 1984-31 Aug 1985
KANSAS UNIV MEDICAL CENTER KANSAS CITY DEPT OF BIOCHEMISTRY
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Out of eleven cDNA clones, we selected three for further characterization by DNA sequence analysis. Two of these clones overlapped by 470 base pairs. Complete DNA sequence of these three clones have been determined. The region sequenced represents about 42 of the dengue virus genome. Examination of the primary nucleotide sequences using computer programs developed for translation in all possible reading frames revealed the presence of long open reading frames spanning the entire length of the cDNA clones. From the DNA sequences, amino acid sequences of segments of putative polyprotein precursor of dengue virus genome were derived. The putative polypeptides derived from the DNA sequence are 885 and 643 amino acids in length. Keywords Hybridization, Dengue viral antigens.
- Genetic Engineering and Molecular Biology