Immunodetection of T-2 Metabolites in Rat Urines after Dermal, Oral, or Intramuscular Exposure to T-2 Toxin
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
T-2 toxin metabolites were detected in rat urines up to 2 weeks after the animals exposure to sublethal dose of T-2 toxin. Urines were assayed for HT-2 or T-2 tetraol by radioimmunoassay RIA with polyclonal antibody. Sensitivity of the RIA was 5 nanogramml for HT2 and 50 nanogramml for T-2 tetraol. Some urines were assayed in parallel with an enzyme-linked immunosorbent assay ELISA developed for T-2 with a monoclonal antibody that cross reacts with HT-2. The sensitivity of the ELISA for detection of HT-2 in rat urines was 250 nanogramml. Urines were diluted 12 or 15 to eliminate high backgrounds, which appeared as false positives in the T-2 tetraol RIA and ELISA assays. T-2 or its metabolites could be detected up to 96 hrs after an im injection of T-2 toxin. In rats exposed orally or dermally, metabolites were detected up to 10 or 16 days, respectively. T-2 equivalents detected by the ELISA correlated with the HT-2 or HT-2-like compounds detected by RIA. Keywords Tusarium, Phytotoxins, Immunoassay.
- Medicine and Medical Research