Screening of Immunoenhancing Drugs with Antiviral Activity Against Members of the Arena-, Alpha-, and Adenoviridae
Annual rept. 15 Jan 1987-14 Jan 1988
ALABAMA UNIV UNIVERSITY
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A series of 18 potential immunomodulators were tested for antiviral activity in mice challenged with lethal doses of either Venezuelan equine encephalitis or mouse adenovirus. Of those tested no compound was able to reduce morality due to Venezuelan equine encephalitis virus. On the other hand, treatment with several of a series of block polymers either singly or in combinations were able to reduce mortality resultant from challenge with mouse adenovirus. While there was no reduction in final mortality with any compound when tested with a Venezuelan equine encephalitis virus challenge, two compound Poly ICLC, CL246.738 were able to significantly extend the time to death of treated mice as compared to controls. In addition, both of those compound as well as Bru-Pel were shown to at least temporarily lower the levels of virus recoverable from treated mice. Finally, a series of assays using Venezuelan equine encephalitis virus-infected cells as targets were developed. These tests were used to show that mice treated with CL246.738 had higher the control levels of NK activity at the time of challenge with virus. On days 1, 3, 1nd 7 after virus infection, more cells were recovered from spleens of CL246,738 treated mice. This increase did not result from a specific increase in the concentration of NK cells. Cytotoxic T cell activity measured seven days after infection showed a similar increase in activity compared to controls. Keywords Antiviral agents Immunochemistry Venezuelan equine encephalitis.