Accession Number:

ADA196772

Title:

Total Triiodothyronine by Fluorescence Polarization Immunoassay (FPIA),

Descriptive Note:

Corporate Author:

SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX

Report Date:

1988-01-01

Pagination or Media Count:

4.0

Abstract:

The determination of total triiodothyronine T3 is a useful adjunct to the more common thyroid function battery consisting of total thyroxine T4, T3 uptake T3U, and thyroid stimulating hormone, TSH. Elevated total T3 values are indicative of hyperthyroid conditions such as T3 toxicosis or Graves disease. Traditionally, radioimmunoassays RIA have been employed for the determination of total T3. Enzyme immunoassays EIA and fluorescence immunoassays FIA have been developed for many of the analytes that formerly were measured using RIA. One variation of this new generation of immunoassays is fluorescence polarization. A fluorescence polarization immunoassay FPIA method for total T3 has been automated by adaptation to the TDx Abbott, Chicago, IL clinical analyzer. The TDx total T3 assay has been evaluated as a replacement for an RIA total T3 procedure. Performance characteristics of the FPIA test are reported here along with comparisons and contrasts with the more commonly used RIA test. Reprints. MJM

Subject Categories:

  • Biochemistry
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE