Accession Number:

ADA196705

Title:

Studies of Altered Response to Infection Induced by Severe Injury

Descriptive Note:

Annual progress rept. 24 Jan 1987-23 Jan 1988

Corporate Author:

MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER

Personal Author(s):

Report Date:

1988-03-01

Pagination or Media Count:

19.0

Abstract:

Twenty-six patients have been studied during this contract year, including 14 trauma and 12 burn patients. Of these patients, 2 burn and 4 trauma patients have succumed to fatal sepsis. Patients showed depressed monocyte MO plasminogen activator PA activity and depressed PHA induced T cell proliferation concomitant with elevated M0 prostaglandin E2 PGE2 production, as well as increased tumor necrosis factor TNF activity and poor prognosis. A post-trauma shift in the ratio of M0 functions. A new bioassay for determination of TNF has been developed this year, and a ELISA technique has been established to detect the M0 PGE2, production. The thermally injured guinea pig model has been applied to pursuing the new prophylactic therapies. In particular we are examining cyclo-oxygenase inhibitor effects on TNF in this model. Overall, this has been a highly productive year. Several other new methods to monitor immunosuppression developed in severely injured patients are being established including an assay for Interleukin-6 and a bioassay for Transforming Growth Factor beta. Several prophylactic drug trails are in progress and we are examining the monocytes from trauma patients receiving ibuprofen and the y 5 antibody against endotoxin. Keywords Monoclonal antibodies, Recombinant DNA, Thermal injury, Infection, Antibody, Leukocytes, Guinea pigs.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE