Accession Number:

ADA196692

Title:

Isolation and Characterization of Chick Epiphyseal Cartilage Matrix Vesicle Proteolipid

Descriptive Note:

Master's thesis

Corporate Author:

AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s):

Report Date:

1988-01-01

Pagination or Media Count:

108.0

Abstract:

Membrane dependent calcification in bacteria involves dicyclohexylcarbodiimide sensitive, ion translocating proteolipids. Since epiphyseal cartilage matrix vesicles appear to support calcification by a similar mechanism, this study was undertaken to determine the chemical composition and dicyclohexylcarbodiimide binding characteristics of matrix vesicle proteolipids. Results indicate that the protein species of matrix vesicle proteolipids differ in molecular weight from those isolated from calcifying bacteria. A subset of matrix vesicle proteolipids exhibit C14- dicyclohexylcarbodiimide binding characteristics comparable to ion translocating proteolipids of bacteria. The similarities between proteolipids from calcifying bacterial and those extracted from calcifying matrix vesicles, indicate that the basic mechanisms of de novo mineralization may proceed by similar paths in these models. Keywords Theses.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE