Gene Regulation of Methanogenesis from Acetate in the Acetotrophic Methane-Producing Archaebacteria
Final rept. 1 Jul 1987-30 Jun 1988
CALIFORNIA UNIV LOS ANGELES DEPT OF MICROBIOLOGY
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The research objectives of the project were to develop genetic and molecular techniques that will permit the detection, isolation, and cloning of genes that are regulated during acetate catabolism in the methanosarcina. These methods should provide a basis for examining the regulation of acetate utilization by the methanogenic Methanosarcina. We have concentrated on three areas in the second year of the contract 1 refining plating techniques and selecting for auxotrophic mutants of Methanosarcina spp., 2 characterizing plasmids from Methanosarcina spp. 3 creating gene libraries of Methanosarcina spp. genomic DNA 4 creating oligonucleotide and antibody probes to probe the chromosonal library for acetoclastic genes.
- Genetic Engineering and Molecular Biology