Accession Number:

ADA196353

Title:

Effect of Electric Fields on Membrane Bound (Na,K)-ATPase

Descriptive Note:

Annual rept. 1 Oct 1987-30 Jun 1988

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF BIOLOGICAL CHEMISTRY

Personal Author(s):

Report Date:

1988-06-30

Pagination or Media Count:

4.0

Abstract:

Sodium, Potassium ATPase of human erythrocytes has been activated by an oscillating electric field of 20 Vcm. The optimum frequency for activation of the Rubidium -pump was 1.0 kHz and for activation of the Na -pump was 1.0 MHz. At 4 C and under the optimum conditions, the net voltage induced, ouabain sensitive Rb uptake was 10 - 20 atto-mole per red cell per hour, and Na efflux was 20 - 30 atto-mole per red cell per hour, depending on erythrocyte samples from different individuals. No electric field stimulated consumption of ATP was detected. Apparently, the enzyme can absorb free energy from the applied electric field for pumping Rb and Na against their respective concentration gradient. We have proposed a mechanism Resonance Electroconformational Coupling to interpret these results. Some feature of these results can also be reproduced by the surface compartmental model of Blank. Keywords Cell membrane, Energy transduction, Signal transduction.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE