Accession Number:

ADA196043

Title:

Protein Adsorption and Its Role in Bacterial Film Development

Descriptive Note:

Annual rept. Feb 1987-Feb 1988

Corporate Author:

DELAWARE UNIV LEWES COLL OF MARINE STUDIES

Report Date:

1988-06-24

Pagination or Media Count:

6.0

Abstract:

Surfaces in seawater are quickly covered by complex organic films which greatly influence subsequent microbial colonization. We have focussed on one predominant component of the organic film, proteins. During this first year, we found that the mechanism of protein adsorption in seawater is similar to that observed in non-marine systems, but unlike non-marine systems, protein desorption can be substantial at high protein concentrations. We also found that the micron-scale distribution of adsorbed proteins varied with different surfaces. Finally, using an immunological assay, we measured dissolved concentrations of ribulose-bisphosphate carboxylase RuBPCase, which is the most abundant protein in nature. RuBPCase comprises about 2 of the total dissolved protein pool, a high percentage considering the large number of possible proteins in seawater. In addition, immunological approaches are being used to examine protein adsorption and degradation in natural microbial films. Keywords Protein adsorption, Bacterial attachment, Fouling.

Subject Categories:

  • Biological Oceanography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE