Accession Number:

ADA193469

Title:

Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.

Descriptive Note:

Annual rept. 15 May 84-14 May 85,

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE

Personal Author(s):

• August, J. T.

Report Date:

1985-07-10

Pagination or Media Count:

27.0

Abstract:

During the second year of this contract, we have purified human erythrocyte acetylcholinesterase to 87 homogeneity by MAb affinity chromatography and size-exclusion HPLC and have obtained sequence data for 100 residues, derived from tryptic, chymotryptic, and V8 peptides. We have used these sequence data to synthesize oligonucleotide probes with which to screen two human cDNA libraries for the gene encoding human AChE. In addition, we have identified a large number of MAb that bind to acetylcholinesterase and we have begun to characterize several of these antibodies that are capable of inhibiting acetylcholinesterase. Keywords Monoclonal antibodies.

Descriptors:

• *ACETYLCHOLINESTERASE
• *ANTIBODIES
• *CLONES
• *CHOLINESTERASE INHIBITORS
• *BIOSYNTHESIS
• CHROMATOGRAPHY
• CODING
• ERYTHROCYTES
• GENES
• HUMANS
• PROTEINS
• PURIFICATION
• SEQUENCES
• PEPTIDES
• CHEMICAL ANALOGS

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE