Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.
Annual rept. 15 May 85-14 May 86,
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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We have studied the binding properties and inhibitory activity of AE-2, a monoclonal antibody which binds to acetylcholinesteraseAChE and inhibits its activity by up to 50. This antibody was compared to C1B7, another inhibitory enzyme. It was determined that these antibodies bind to different sites on AChE these sites were distinct from the active site and from the binding sites for a number of anticholinergic drugs, including DFP, propidium, endrophonium and neostigmine. A kinetic analysis of inhibition by AE-2 and C1B7 suggested that their inhibition of AChE was non-competitive and allosteric.