Accession Number:

ADA193383

Title:

Molecular Biology of Archaebacteria.

Descriptive Note:

Annual rept. Apr 87-Mar 88,

Corporate Author:

BRITISH COLUMBIA UNIV VANCOUVER DEPT OF BIOCHEMISTRY

Personal Author(s):

• Dennis, Patrick P.

Report Date:

1988-03-31

Pagination or Media Count:

7.0

Abstract:

Genomic restriction fragments from Halobacterium cutirubrum Hcu and Sulfolobus acidocaldorius Sac encoding ribosomal protein L11e, L1e, L10e and L12e were cloned and sequenced. The sequences were analyzed and the reading frames identified. The deduced L10e and L12e protein sequences were aligned with eubacterial and eucaryotic proteins and their structure-function and evolutionary relationships were defined. The N-terminal amino acid sequence of the purified superoxide dismutase enzymes from Hcu was determined, an oligonucleotide was prepared and the gene was cloned and sequenced. The sequence has been aligned to other SOD sequences. The gene transcripts have been characterized and the regulation of transcription induction by oxygen radicals has been demonstrated and characterized.

Descriptors:

• *BACTERIA
• *MOLECULAR BIOLOGY
• CHEMICAL RADICALS
• CLONES
• ENZYME CHEMISTRY
• ENZYMES
• GENES
• OXIDOREDUCTASES
• OXYGEN
• PURIFICATION
• SEQUENCES
• SUPEROXIDES
• GENETICS
• PROTEINS

Subject Categories:

• Genetic Engineering and Molecular Biology
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE