Liquid Storage at 4 deg C of Previously Frozen Red Cells
LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA
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Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 deg C. The red cells ere thawed, deglycerolized, and resuspended in a final was solution, ADSOL Fenwal Laboratories, or an additive solution AS containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 deg C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post-thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post-thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.
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