Accession Number:

ADA193265

Title:

Liquid Storage at 4 deg C of Previously Frozen Red Cells

Descriptive Note:

Journal article

Corporate Author:

LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s):

• Moore, G. L.
• Ledford, M. E.
• Mathewson, P. J.
• Hankins, D. J.

Report Date:

1987-12-01

Pagination or Media Count:

3.0

Abstract:

Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 deg C. The red cells ere thawed, deglycerolized, and resuspended in a final was solution, ADSOL Fenwal Laboratories, or an additive solution AS containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 deg C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post-thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post-thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.

Descriptors:

• *BLOOD STORAGE
• ACID CITRATE DEXTROSE
• ADDITIVES
• ADENINE
• ADENOSINE PHOSPHATES
• BLOOD
• BLOOD BANKS
• BLOOD CHEMISTRY
• CIVILIAN PERSONNEL
• COSTS
• ERYTHROCYTES
• FREEZING
• GLUCOSE
• HUMANS
• LYSIS
• MANNITOL
• METABOLISM
• PHOSPHATES
• REPRINTS
• SOLUTIONS(MIXTURES)
• STOCKPILES

Subject Categories:

• Medical Facilities, Equipment and Supplies

Distribution Statement:

APPROVED FOR PUBLIC RELEASE