Molecular Mechanisms of Cytopathogenicity of Primate Lymphotropic Retroviruses: Relevance to Treatment and Vaccine for AIDS.
Annual rept. 29 Sep 86-28 Sep 87,
BIOTECH RESEARCH LABS INC ROCKVILLE MD
Pagination or Media Count:
The molecular basis of infectivity, cytopathogenicity and genomic activation of Human Immunodeficiency Virus Type 1HIV-1 was investigated by generating deletion mutations in sor, 3orf, 5 region, and LTR. These studies show that the sor gene is crucial for the generation of infectious virus. Analysis of 3orf indicate that deletions in the amino terminus which overlaps the carboxy terminus of gp41 result in significant reduction in viral propagation. Studies with the LTR sequency have delineated an enhancer activity, an sp-1 bending site, and a tat response region TAR. Genomic clones containing the integrated provirus of Simian T-Cell Lymphotorpic Virus Type IIIAfrican Green Monkey STLV-IIAGM and HIV-2NIH-Z have been isolated. Their nucleic acid sequence has been determined and subjected to computer analysis. A Comparison of the nucleotide sequences of these two new viral isolates with HIV-1 indicates that these new retroviruses are structurally similar to HIV-1 and belong to the same class of viruses as HIV-1. STLV-III and HIV-2NIH-Z exhibit a greater homology among themselves than with HIV-1. A study of biologically active forms of these viruses will aid in understanding why these new viruses are non-pathogenic. Keywords Vaccines, Acquired immune deficiency syndrome, Virus diseases, Immunosuppression, Deoxyribonucleic acids.
- Genetic Engineering and Molecular Biology