Pathogenesis and Prevention of Acute Renal Failure.
Annual rept. 2 Sep 86-3 Sep 87,
COLORADO UNIV AT DENVER MEDICAL CENTER
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Several in vitro studies on the isolated perfused kidney IPK and renal tubules freshly isolated from the rate were performed to evaluate the mechanism of oxygen deprivation injury and protection. Emopamil, a calcium channel blocker, prevented both warm and cold ischemia in the IPK. Cold perfusion alone is also of benefit and can be enhanced by verapamil. Atrial peptide III the atrial natriuretic factor ANF exerts protection to the IPK from renal ischemia injury as well as in the whole animal. Using NMR, the protection appears to involve preservation of adenine nucleotides and prevention of intracellular acidosis. Furthermore, fructose diphosphate is also protective possibly by providing glycolytic intermediates. The tubule studies were characterized by technical improvements in preparative and incubation techniques and development of methods to ensure anoxic injury and its sequelae. Keywords Acute renal failures, Hypoxia, Nuclear magnetic resonance.