Accession Number:
ADA190155
Title:
Procedures for Detoxification of Brevetoxins PbTx-2 and PbTx-3 (Neurotoxins from the Florida Red Tide Dinoflagellate Ptychodiscus brevis)
Descriptive Note:
Progress rept.
Corporate Author:
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Personal Author(s):
Report Date:
1987-11-10
Pagination or Media Count:
20.0
Abstract:
Procedures fordetoxification of the brevetoxins PbTx-2 and PbTx-3 were investigated using the Japanese madaka Oryzias latipes as the assay model. PbTx-2 was 100 lethal to these fish at concentrations above 70 ngml in the aquarium water, while PbTx-3 was less potent. Treatment with 0.5 ml 0.1 N naOH for 10 min detoxified a lethal dose of either PbTx-3 or PbTx-3 to below the detection limits of this assay 10 ngml. Decreasing NaOH concentration required correspondingly longer incubation times. Potency of both toxins was also destroyed by incubation at 500 C for 10-15 min. Steam autoclaving at 122 C 30 min, 18 psi was not sufficient to detoxify brevetoxins. These results show that washing or soaking in a dilute NaOH solution will decontaminate laboratory glassware and equipment and render it safe for normal handling. Disposable waste can be either soaked in a NaOH solution prior to disposal or burned in an incinerator with a combustion chamber of at least 500 C. Steam autoclaving, however, is not a viable method of decontamination.
Descriptors:
Subject Categories:
- Toxicology