Accession Number:

ADA190155

Title:

Procedures for Detoxification of Brevetoxins PbTx-2 and PbTx-3 (Neurotoxins from the Florida Red Tide Dinoflagellate Ptychodiscus brevis)

Descriptive Note:

Progress rept.

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

• Poli, Mark A.

Report Date:

1987-11-10

Pagination or Media Count:

20.0

Abstract:

Procedures fordetoxification of the brevetoxins PbTx-2 and PbTx-3 were investigated using the Japanese madaka Oryzias latipes as the assay model. PbTx-2 was 100 lethal to these fish at concentrations above 70 ngml in the aquarium water, while PbTx-3 was less potent. Treatment with 0.5 ml 0.1 N naOH for 10 min detoxified a lethal dose of either PbTx-3 or PbTx-3 to below the detection limits of this assay 10 ngml. Decreasing NaOH concentration required correspondingly longer incubation times. Potency of both toxins was also destroyed by incubation at 500 C for 10-15 min. Steam autoclaving at 122 C 30 min, 18 psi was not sufficient to detoxify brevetoxins. These results show that washing or soaking in a dilute NaOH solution will decontaminate laboratory glassware and equipment and render it safe for normal handling. Disposable waste can be either soaked in a NaOH solution prior to disposal or burned in an incinerator with a combustion chamber of at least 500 C. Steam autoclaving, however, is not a viable method of decontamination.

Descriptors:

• *DECONTAMINATION
• *DINOFLAGELLATA
• DETECTION
• MODELS
• GLASS
• LIMITATIONS
• VIABILITY
• NERVOUS SYSTEM
• INCUBATION
• WASTES
• DISPOSAL
• INCINERATORS
• STEAM
• FLORIDA
• LETHAL DOSAGE
• TOXINS AND ANTITOXINS
• FISHES
• DETOXIFICATION
• BIOASSAY
• DISPOSABLE EQUIPMENT
• SODIUM HYDROXIDE
• AUTOCLAVES

Subject Categories:

• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE