Accession Number:

ADA190151

Title:

Alteration of Multiple Cell Membrane Functions in L-6 Myoblasts by T-2 Toxin: An Important Mechanism of Action.

Descriptive Note:

Interim rept.,

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

Report Date:

1986-06-04

Pagination or Media Count:

31.0

Abstract:

Recent studies suggest that T-2 toxin interacts with cell membranes and alters membrane function. This study was done to assess the effect of T-2 toxin on a broad range of cell membrane functions in L-6 myoblasts. The following parameters were assessed after exposure to T-2 toxin for 10 min the uptake of calcium,rubidium, and glucose the uptake of leucine and tyrosine and incorporation into protein the uptake of thymidine and incorporation into DNA and residual cellular lactate dehydrogenase LDH as a measure of cell membrane integrity. The effects of T-2 toxin on these parameters were the minimal effective concentration MEC of T-2 toxin that caused a reduction in uptake of calcium and glucose was 4 pgml. The MEC for reduction of uptake of leucine and tyrosine and their incorporation into protein was 4 pgml. Thymidine uptake and incorporation into DNA showed a biphasic response with an increase at 0.4 pgml and a reduction at 4 pg for uptake and 40 pgml for incorporation. 4 Intracellular LDH was reduced at 4 ngml. 5 Calcium efflux was reduced after 1-, 5-, adn 15-min exposure to T-2 toxin in a concentration of 40 ngml. T-2 toxin appears to have multiple effects on cell membrane function including effects on amino acid, nucleotide, and glucose transporters, as well as calcium and potassium rubidium channel activities.

Subject Categories:

  • Toxicology
  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE