Synthetic Peptide Vaccines for the Control of Arenavirus Infections
Final rept. 1 Dec 1982-31 Jul 1986
SCRIPPS CLINIC AND RESEARCH FOUNDATION LA JOLLA CA
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In our original research contract proposal C3013 entitled Synthetic Peptide Vaccines for the Control of Arenavirus Infections, we proposed to develop synthetic peptide based approaches. In addition, we proposed to investigate the potential of immune reagents, particularly monoclonal antibodies to control and modify the course of ongoing disease in animals using lymphocytic choriomeningitis virus infection as a safe and accurate paradigm for the pathogenic human arenaviruses. We have accomplished the goals set forth in the original proposal. THe cDNA sequence of the S RNA segment of LCM virus has been determined and the protein sequences of the structural proteins NP, GP-1 and GP- 2 have been deduced. Synthetic peptides corresponding to sequences within the structural proteins have been made and corresponding antisera prepared against them in rabbits. These antisera have been used to map topographically and structurally important domains on the viral glycoproteins. A large panel of monoclonal antibodies to the LCMV glycoprotein has been mapped topographically on the LCMV glycoproteins and both unique and conserved neutralizing epitopes defined. Finally, the sequences of the LCMV and other arenaviruses have been aligned and compared and common structural features of each determined in order to define regions of functional andor immunological homology. Keywords Vaccines DiagnosisMedicine.