Accession Number:

ADA188171

Title:

Degradation of Albumin by Trypsin: Potential Application of High-Performance Liquid Chromatography (HPLC) and Differential Scanning Calorimetry (DSC) for Monitoring the Degradation of Proteinaceous Toxins by Proteolytic Enzymes.

Descriptive Note:

Technical rept. Dec 85-Feb 86,

Corporate Author:

CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD

Personal Author(s):

• Diamandaras, Penelope
• White, William E.
• Elashvilli, Ilya

Report Date:

1987-08-01

Pagination or Media Count:

19.0

Abstract:

Albumin was selected as a model protein for developing assay systems for monitoring degradation by proteolytic enzymes. Using a diode array detector, high-performance liquid chromatography was ideal for following albumin digestion by trypsin. The albumin peak gradually diminished and was replaced by several peaks corresponding to shorter peptides. In contrast, differential scanning calorimetry was of no value in following the disappearances of the protein. A thermal transition resulting from some confirmational change occurred at 52 C but did not change upon digestion of the protein.

Descriptors:

• *ALBUMINS
• *DIGESTION(BIOLOGY)
• *TRYPSIN
• ARRAYS
• CALORIMETRY
• DEGRADATION
• DETECTORS
• DIODES
• LIQUID CHROMATOGRAPHY
• MODELS
• MONITORING
• PEAK VALUES
• PEPTIDE HYDROLASES
• PEPTIDES
• PROTEINS
• SCANNING
• THERMAL PROPERTIES
• TRANSITIONS
• ENZYMES
• BACTERIAL TOXINS
• PHYTOTOXINS
• BIOASSAY

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE