Evaluation of the Erythrocyte Malondialdehyde (MDA) Release Assay.
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
Pagination or Media Count:
In the past, vitamin E status has most commonly been measured utilizing a static index such as the plasma concentration. However, since vitamin E circulates with plasma lipids, the plasma vitamin E concentration is considered to be unreliable in some clinical situations. For example, in patients with chronic cholestasis or hyperlipidemia, it has been suggested that the increased lipids in these disorders cause vitamin E to partition from the RBC membrane and other tissues. Thus, a falsely elevated plasma vitamin E level is observed and is not reflective of total body vitamin E. We evaluated a functional approach for assessing vitamin E status which circumvents this partitioning problem. In evaluating the MDA release assay we performed anticoagulant, stability, and precision studies. For clinical studies, we examined vitamin E sufficient adults, vitamin E sufficient and deficient children, and hyperlipidemic males. Results were compared with several static indices including plasma vitamin E concentration and plasma total lipid concentration. We have refined the MDA release assay to the point where it can be a useful clinical laboratory procedure.
- Anatomy and Physiology