Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.
Annual progress rept. 1 Jan 83-1 Jan 84,
COLORADO STATE UNIV FORT COLLINS DEPT OF MICROBIOLOGY AND ENVIRONMENTAL HEALT H
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Studies were conducted to compare the efficiency of oral infection, replication and oral transmission of dengue- 1 and dengue-4 candidate vaccine viruses and their respective parent viruses in vector mosquitoes. The dengue-1 candidate vaccine virus, TP-56 was not altered in its ability to infect and to replicate in Aedes aegypti or Aedes albopictus mosquitoes. Oral infection and dissemination rates with the vaccine virus were as high or higher than for the parent virus. Transmission rates of vaccine infected mosquitoes were comparable to parent infected mosquitoes. The dengue-1 candidate vaccine virus 45AZ5, was found to be phenotypically stable after up to 3 passages in ae, aegypti or Ae, albopictus mosquitoes. Studies are continuing on its ability to infect, replicate in, and be transmitted by mosquitoes. The dengue-4 candidate vaccine virus, H-241, Lot 1, was less efficient than its parent virus in infection and replication in both Ae. aegypti and Ae. albopictus mosquitoes. After oral infection, the vaccine virus replicated at a slower rate and to a lower titer than the parent virus in both mosquito species. Limited transmission data indicates the vaccine virus is either unable to or greatly reduced in its ability to be transmitted by mosquitoes compared to parent virus.