Physiologic Aging of Mature Porcine Erythrocytes: Effects of Various Metabolites, Antimetabolites, and Physical Stressors
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX
Pagination or Media Count:
Red blood cells were collected from Landrace x Duroc pigs in pooled and single batches. The RBC were stored for 24 hours to 20 days and exposed to 1 or more chemical and physical stressors. The chemicals were pyruvate, lactate, inosine, concanavalin A-luminol-bovine serum albumin conjugate, hydrogen peroxide, L-mimosine, and 3-amino-L-tyrosine. Physical stressors included thermogenic microwave radiation 2,450 MHz, mean specific absorption rate of 91 Wkg and conventional heating with hot air or hot-water bath. Heating erythrocytes to 43 C for 10 minutes with microwaves or hot air did not significantly increase hemolysis, compared with hemolysis of RBC at 4 C controls. Pyruvate or lactate did not affect the RBC under these conditions. Preincubation with 1.49 mM 3-amino-L-tyrosine totally inhibited the increase in binding with pyruvate. Therefore, the effectiveness of 3-amino-L-tyrosine for protecting natural membrane components from oxidation is related to its thermoprotective properties. The luminol-bovine serum albumin binding assay was a sensitive, simple technique for detecting oxidative physiologic aging of porcine RBC.
- Stress Physiology