Prevention of Canine Graft-versus-Host Disease (GVHD).
Technical rept. 1 Jun 83-1 Feb 85,
FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA
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This work was aimed at the ultimate goal of solving the two major problems of marrow transplantation resistance to grafts, and graft-versus-host disease. Work done towards these goals has included the establishment of a laboratory specialized in the production and characterization of murine monoclonal antibodies to canine mononuclear cells. Antibodies with a broad range of specificities have been generated and have served to establish a first breakdown of canine mononuclear cells into functional subsets, e.g., helper and suppressor cells. A large number of antibodies generated against cells from other species have been screened for cross-reactivity with canine cells with remarkable success, in particular antibodies against monomorphic framework structures of Ia. Antibodies, including cross-reactive ones, have been used in a model of autologous marrow transplantation, and strong evidence for the presence of Ia antigens on pluripotent hematopoietic stem cells has been obtained. The rate of hematopoietic engraftment in recipients of SLA-incompatible grafts was increased from 5 to 50 when recipients were treated in vivo with an antibody to a monomorphic Ia framework determinant antibody 7.2.
- Medicine and Medical Research
- Animal Husbandry and Veterinary Medicine