Genetics, Structure and Functional Features of the RNA Modification Enzyme, tRNA psi Synthase I.
Summary rept. 1 Feb 84-31 Jan 85,
CALIFORNIA UNIV OAKLAND NAVAL BIOSCIENCES LAB
Pagination or Media Count:
Nucleoside modifications are significant structural components of most classes of cellular Ribonucleic Acids. About 50 RNA modifications are known, varying in complexity from simple base or sugar methylations to multifunctional heterocyclic groups. The use of RNA modification enzymes as probes of protein-nucleic acid interaction has been limited by the lack of pure enzymes of this type. Our goal is to develop the pseudouridine modification system for this purpose, by identifying the enzymes, genes and molecular interactions involved in the synthesis of this nucleoside.
- Genetic Engineering and Molecular Biology