Gene Regulation of Methanogenesis from Acetate in the Acetotrophic Methane Producing Archaebacteria.
Annual rept. 1 Apr 86-31 Jun 87,
CALIFORNIA UNIV LOS ANGELES DEPT OF MICROBIOLOGY
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The research objectives of the project are to develop genetic and molecular techniques that will permit the detection, isolation, and cloning of genes that are regulated during acetate catabolism. These studies should provide a firm basis for understanding the regulation of acetate utilization in the methanogen, Methanosarcina acitivorans. We have concentrated on three areas of study in the first year of the contract. They are 1development of cell plating methods for the methanosarcina, 2 screening and isolation of plasmids from the acetogenic methanogens, and 3 construction of gene libraries for M. acetivorans. It is anticipated that techniques developed in these studies will facilitate genetic study of other methanogenic organisms. Keywords Archaebacteria, Methanogens, Acetate Utilization, Genetic Regulation, Plasmids, Nutrition.