Monoclonal Antibodies that Recognize Proteins Unique to Somatic Embryos of Daucus carota.
Summary rept. 1 Oct 84-30 Sep 85,
CALIFORNIA UNIV OAKLAND NAVAL BIOSCIENCES LAB
Pagination or Media Count:
We have developed a panel of monoclonal antibodies Mabs to several antigens of Daucus carota somatic embryos. We prepared over 2400 hybridoma cultures from splenocytes of Balbc mice immunized with lyophilized somatic embryo cells. The MAbs were screened on embryo, callus cell, and chloroplast extracts using an automated sampling system and computer-assisted analyses. Most of the MAbs reacted with antigens common to embryo and callus cells, nut MAbs from al least 5 hybridoma lines recognized single antigens unique to the somatic embryos. Other lines produced antibodies to chloroplast antigens, including the light-harvesting chlorophyll protein LHCP.
- Genetic Engineering and Molecular Biology