Bimane Derivatives as Fluorescent Probes for Biological Macromolecules.
Final rept. Nov 83-Dec 86,
TEL-AVIV UNIV (ISRAEL) DEPT OF CHEMISTRY
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Syn-9, 10-Dixoabimanes 1,5-diazabicyclo3.3.0octa-3,6-diene-2,8-diones are photostable, highly fluorescent compounds which can be attached to biological macromolecules as fluorescent labels. Syntheses of the important new intermediates for labeling agents, syn-tricyclic bimanes, RuCn RCH3 or C1 and n 0.1,2,3 have been developed. The conversion of the compounds with n2 into a diene has been accomplished, successfully raising the adsorption coefficient. However, interesting and surprising physical behavior conspired to drastically diminish the fluorescence quantum yield. The discovery of several routes to unsymmetrically substituted bimanes prepared by nucleophilic displacement on bromoalkyl derivatives has revealed new classes of labeling agents for macromolecules. These include a cross-linking agent.
- Organic Chemistry