Accession Number:

ADA183795

Title:

The Quantitation of Pyridostigmine in Plasma by HPLC (High Pressure Liquid Chromatographic).

Descriptive Note:

Final rept. 1 Apr-30 Nov 86,

Corporate Author:

LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s):

• Ryabik,John R.
• Ho,Bert

Report Date:

1987-07-01

Pagination or Media Count:

17.0

Abstract:

A normal-phase HPLC method utilizing ion-pair extraction for the quantitation of pyridostigmine in plasma is described. The limit of quantitation was 2 ngml plasma intra-day precision with coefficient of variation CV of 1.4 50 ngml and inter-day precision of 2.6 50 ngml were obtained. The method was designed for use in pharmacokinetic studies with the Rhesus monkey, but has been successfully adapted and applied to the analysis of plasma from rats dosed with pyridostigmine in the feed. Keywords Blood plasma Acetycholinesterase, Pharmicokinetics.

Descriptors:

• *BLOOD PLASMA
• *LIQUID CHROMATOGRAPHY
• *TOXICOLOGY
• *ANTIDOTES
• HIGH PRESSURE
• LIQUIDS
• PHARMACOKINETICS
• RATS
• RHESUS MONKEYS
• CHOLINESTERASE INHIBITORS
• ACETYLCHOLINESTERASE
• ORGANOPHOSPHATES
• POISONING

Subject Categories:

• Pharmacology
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE