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Recent Advances in Multidimensional Fluorescence Spectrometry.
EMORY UNIV ATLANTA GA DEPT OF CHEMISTRY
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This review article discusses some of the latest developments and applications in the field of multidimensional fluorescence techniques. Numerous important advances have been made in the past few years, mostly due to improvements in automated instrumentation. In addition, the increased use of multiple parameters has provided recognized advantages. The multiparametric term has been used most often in systems only where the fluorescence intensity is observed as a function of two variables e.g. excitation and emission. We review the utility of other parameters, including circular dichroism, fluorescence lifetime and chromatographic retention time, in enhancing the fluorescence measurement. In addition, we discuss powerful algorithms which have been developed to extract meaningful information from various types of multidimensional fluorescence data. Keywords multidimensional luminescence chromatography time resolved luminescence spectroscopy excitation emission matrix.
APPROVED FOR PUBLIC RELEASE