Accession Number:

ADA183229

Title:

Molecular Cloning of the Human Genes(s) Directing the Synthesis of Nervous System Cholinesterases.

Descriptive Note:

Annual rept. no. 3, 1 Nov 84-31 Oct 85,

Corporate Author:

WEIZMANN INST OF SCIENCE REHOVOT (ISRAEL) DEPT OF NEUROBIOLOGY

Personal Author(s):

• Soreq,Hermona

Report Date:

1985-12-01

Pagination or Media Count:

56.0

Abstract:

Complementary DNA cDMA and genomic DNA clones coding for human cholinesterase ChE were selected from phage DNA libraries by synthetic oligodeoxynucleotides coding for the organophosphate binding site of ChE and were characterized, sequenced and expressed within bacterial cells to yield AChE-like proteins. The Xenopus oocytes microinjection bioassay for translation of ChEmRNA has been improved and refined for studying the post-translational processing of nascent ChE molecules. ChE polymorphism and auto-immune anti-ChE antibodies were studied. Keywords Acetylcholinesterase Pseudocholinesterase Immunoblotting Poisoning Cholinesterase inhibiters.

Descriptors:

• *DEOXYRIBONUCLEIC ACIDS
• *CLONES
• *MOLECULES
• *ACETYLCHOLINESTERASE
• CHOLINESTERASE
• HUMANS
• GENETIC ENGINEERING
• NERVOUS SYSTEM
• BACTERIOPHAGES
• MUTAGENS
• CODING
• CODING
• GENES
• HUMANS
• MOLECULES
• ORGANOPHOSPHATES
• SITES
• POISONING
• CHOLINESTERASE INHIBITORS
• SYNTHESIS(CHEMISTRY)
• BIOCHEMISTRY
• LIBRARIES
• POLYMORPHISM

Subject Categories:

• Genetic Engineering and Molecular Biology
• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE