Cytochemical Studies on Acetylcholine Synthesis and Metabolism in the Vestibular Cerebellum.
Final rept. 1 Sep 82-31 Aug 86,
HARVARD UNIV CAMBRIDGE MA
Pagination or Media Count:
An immunocytochemical study of the cerebellar cortex was carried out in order to determine the distribution and correlations of several neuroactive agents, vis., acetylcholine, taurine, gamma aminobutyric acid, and several peptides. Purkinje cells containing acetylcholine and its metabolic enzymes are most common in the flocculondular lobe and they are randomly distributed without a clear pattern. In contrast Purkinje cells containing the enzymes glutamic acid decarboxylase for GABA, or cystein-sulfinic acid decarboxylase for taurine or the peptide motilin are organized into microzones, longitudinal strips that alternate with strips containing other chemicals. Some Purkinje cells contain more than one neuroactive agent. These chemically characterized strips are closely matched with the location of mossy fibers representing the distal parts of the lower limbs. Keywords Immunocytochemistry.
- Anatomy and Physiology