Human Immune Responses to Dengue Viruses.
Final rept. 1 Sep 82-30 Jun 86,
MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA
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The purpose of this contract was to analyze the immune responses to dengue infections. During the past few years we have focused on three immune responses lysis of dengue virus-infected cells by peripheral blood mononuclear cells PBMC of non-immune donors antibody-dependent complement-mediated lysis of dengue virus-infected cells and interferon induction from PBMC by dengue virus. Results indicate that both PBMC-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity ADCC can cause significant lysis of dengue-infected Raji cells The PBL Peripheral Blood Lymphocytes that are active in lysing dengue virus-infected cells are heterogeneous and are contained in Leu11 and T3 susets. Results also indicated that PBL produced IFN in response on dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue virus-infected cells. The ability of dengue in infected cells. Treatment of monocytes with IFN decreased the yield of infectious virus more than 99 and the percentage of dengue-antigen positive cells by 98. These results suggest that IFNs produced by PBL in response to dengue virus-infected cells may have an important role in controlling dengue infection and in the pathogenesis of dengue hemorrhagic fever and shock syndrome.
- BLOOD CELLS
- GROUP B ARBOVIRUSES
- HEMORRHAGIC FEVERS
- INDUCTION SYSTEMS
- INFECTIOUS DISEASES
- SIGNS AND SYMPTOMS
- Medicine and Medical Research